Advanced course on 3D microscopy imaging of plant tissues and image processing
| FS25_3DMircoImg | |
| 14.04.2025 - 17.04.2025 | |
| 4 days | |
|
|
|
Beginn Anmeldefrist: 01.11.2024 Ende Anmeldefrist: 31.03.2025 |
|
| Extern | |
| 10 | |
| 10 | |
| Students enrolled in PSC PhD Programs: CHF 0 LSZGS PhD students: CHF 0 All others: CHF 300 | |
| This course addresses the challenges of subcellular localization of fluorescent compounds in intact plant tissues and organs. Key issues include the high refractive index of fresh tissues, sample thickness, and stress-induced autofluorescence in dissected samples, alongside classical concerns such as photobleaching and phototoxicity. These factors complicate high-resolution and time-lapse imaging of fluorescent reporter proteins. Participants will explore microscopy techniques tailored to specific applications, receive practical tips for optimizing imaging conditions, and engage in hands-on sessions with confocal laser scanning microscopy and multiphoton imaging. Emphasis will be placed on customizing acquisition parameters to achieve maximum resolution while balancing speed, viability, photobleaching, and signal diffraction in fresh versus fixed Arabidopsis tissues. Additionally, the course will introduce image data management and analysis techniques, including 3D volume rendering, image segmentation, and quantitative information extraction for statistical analysis using tools like Fiji, Imaris, and Biom3D, an ML-based segmentation tool. | |
| Célia Baroux (coordinator), Department of Plant and Microbial Biology, University of Zürich & Christophe Tatout, University of Clermont Auvergne, France | |
| 1 | |
| PhD Postdocs if places available | |
| The course is designed ONLY for students with a PRIOR experience in CSLM imaging and whose research involves (or will involve) 3D imaging in plant tissues. Students should have a good understanding of fluorescence microscopy and confocal imaging principle. A selection of must-read articles will be provided one month before course start. | |
| English | |
| In order to complete the course and gain their credit point, students will be required to attend the whole course and participate actively. | |
By registering you agree to the PSC course terms and conditions AGBs | |
| Cancellation of a course registration should be arranged with the course coordination office phdecology@ieu.uzh.ch and is possible free of charge up to 2 weeks before the course starts. Later cancellations and failure to attend or incomplete attendance without documented justification will incur a fee of 200 CHF. | |
| Dr. Bojan Gujas (psc_phdprogram@ethz.ch) | |
| FS25_Advanced course on 3D microscopy imaging of plant tissues and image processing.pdfvertical_align_bottom |